近日，司法鑒定科學研究院嚴慧課題組湯以綾老師，使用IPHASE品牌產(chǎn)品：II相代謝穩(wěn)定性試劑盒（UGTs）在《Journal of Pharmaceutical and Biomedical Analysis》權-威期刊上發(fā)表文章《Metabolism of dipentylone in zebrafish and human liver microsomes determined by liquid chromatography–high resolution mass spectrometry 》，影響因子3.4！

本論文中，利用LC-HRMS分析dipentylone在斑馬魚和HLM體內和體外的代謝，并成功鑒定了代謝產(chǎn)物及其主要代謝途徑，選擇了合適的代謝物作為生物標志物。

摘要

The consumption of novel psychoactive substances (NPS) is exceedingly prevalent in society, as these substances are sold and distributed as “legal highs.” One novel synthetic cathinone emerging in the market is 1-(1,3-ben-zodioxol-5-yl)–2-(dimethylamino) pentan-1-one (dipentylone). The goal of this work was to study the in vivo and in vitro metabolism of dipentylone in zebrafish and human liver microsomes (HLMs) by liquid chromatography-high resolution mass spectrometry (LCHRMS). The zebrafish and HLM samples contained 14 dipentylone metabolites, specifically 12 phase I metabolites and 2 phase II metabolites. The main metabolic pathways included monohydroxylation (M1 and M2), N-dealkylation (M3), hydroxylation of the aromatic ring and dealkoxylation of M3 (M4), O-dealkylation (M5), N-dealkylation of M5 (M6), reduction of carboxide (M7), monohydroxylation of M5 (M8), dehydrogenation (M9), dealkoxylation (M10), N-dealkylation of M10 (M11), dealkoxylation of M9 (M12), glucuronidation of M5 (M13), and sulfation (M14). The monohydroxylated metabolite (M2) can be recommended as metabolic markers for dipentylone. This study is the first to identify a target compound for monitoring the abuse of dipentylone and to determine the essential chemical structure of the metabolites for urther toxicological research.

完整版文獻可在【IPHASE】公眾號后臺留言【獲取文章】

再次恭喜文獻發(fā)表，以及對我司產(chǎn)品的認可，希望以上文獻能幫助大家了解目前研究進展及我們的核心技術，歡迎各位新老客戶聯(lián)系我們咨詢、提出意見，愿我們的努力成果與您的科研碰撞出不一樣的火花！

發(fā)    文    章    得    獎    勵

凡使用本公司產(chǎn)品，在國內及國際刊物上發(fā)表論文（論文發(fā)表日起一年內），并注明產(chǎn)品屬于IPHASE BIOSCIENCES CO,LTD / 匯智和源生物技術（蘇州）有限公司所有，即可申請獎勵。根據(jù)發(fā)表刊物影響因子不同，給予不同金額獎品：

非SCI論文及IF≤5分，500元禮品；

5分＜IF≤8分 800元；

8分＜IF≤10分 1000元；

IF≥10分 2000元；

注：禮品卡也可兌換同等金額產(chǎn)品購買抵用券；

活動多多，禮品豐厚，快來參與吧！

關    于    我    們

匯智和源，致力于為創(chuàng)新藥研發(fā)企業(yè)及生命科學研究機構提供高品質的生物試劑，IPHASE為公司核心品牌，品牌宗旨“Innovative Reagents For Innovative Research”。